Sandbox000

2D-Mass chromatogram (Condition 1)

Instrument	Shimadzu LC-IT-TOF MS ESI (Negative ion mode)
Column	Waters Atlantis T3 (2.1 mm x 150 mm)
Column Temperature	40℃
Solvent A	5 mM Ammonium acetate solution
Solvent B	acetonitrile
Gradient	10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
Source voltage	– 3.5 kV
Capillary temperature	200 °C
Nebulizer gas	1.5 l/min

Retention Time (min)	Compounds	Molecular formula	(M-H)- ions
	12.60	Liquiritin	C₂₁H₂₂O₉
417.1191	12.85	Liquiritin apioside	C₂₆H₂₂O₉
549.1614	13.41	Licoricesaponin G2	C₄₂H₆₂O₁₇
837.3914	14.10	Glycyrrhizin	C₄₂H₆₂O₁₆
821.3965	14.26	Licoricesaponin A3	C₄₈H₇₂O₂₁
983.4493	14.47	Licoricesaponin H2	C₄₂H₆₂O₁₆
821.3965	14.92	Licoricesaponin E2	C₄₂H₆₀O₁₆
819.3809	16.12	Licorice glycoside E	C₃₅H₃₅NO₁₄
692.1985	[[]]