LCMS:Smilacis Rhizoma

From Metabolomics.JP
(Difference between revisions)
Jump to: navigation, search
 
(3 intermediate revisions by one user not shown)
Line 1: Line 1:
==TIC==
+
{{LCMS/Header}}
[[Image:Smilacis LC-MS.jpg|thumb|700px|center|LC-MS TIC of extracts]]
+
{{CrudeDrug/TIC
 +
|TICFile=Smilacis LC-MS
 +
}}
  
==Analytical conditions==
+
{{CrudeDrug/LCMS/Pos
{| class="wikitable" style="width:80%"
+
|PInstrument =Shimadzu LC-IT-TOF MS ESI
|-
+
|PColumn    =Waters Atlantis T3 (2.1 mm x 100 mm, 3 micron)
| Instrument || Shimadzu LC-IT-TOF MS ESI (Positive ion mode)
+
|PTemperature=40
|-
+
|PSolventA  =5 mM Ammonium acetate solution
| Column || Waters Atlantis T3 (2.1 mm x 100 mm, 3 micron)
+
|PSolventB  =acetonitrile
|-
+
|PGradient  =10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
| Column Temperature|| 40℃ 
+
|PVoltage    =4.5
|-
+
|PCapillary  =200
| Solvent A || 5 mM Ammonium acetate solution
+
|PGas        =1.5
|-
+
}}
| Solvent B || acetonitrile
+
|-
+
| Gradient || 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
+
|-
+
| Source voltage ||  4.5 kV
+
|-
+
| Capillary temperature || 200 °C
+
|-
+
| Nebulizer gas || 1.5 l/min
+
|}
+
  
{| class="wikitable" style="width:80%"
+
{{CrudeDrug/LCMS/Neg
|-
+
|NInstrument =Shimadzu LC-IT-TOF MS ESI
| Instrument || Shimadzu LC-IT-TOF MS ESI (Negative ion mode)
+
|NColumn    =Waters Atlantis T3 (2.1 mm x 150 mm)
|-
+
|NTemperature=40
| Column || Waters Atlantis T3 (2.1 mm x 150 mm)
+
|NSolventA  =5 mM Ammonium acetate solution
|-
+
|NSolventB  =acetonitrile
| Column Temperature|| 40℃ 
+
|NGradient  =10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
|-
+
|NVoltage    =-3.5
| Solvent A || 5 mM Ammonium acetate solution
+
|NCapillary  =200
|-
+
|NGas        =1.5
| Solvent B || acetonitrile
+
}}
|-
+
| Gradient || 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
+
|-
+
| Source voltage || – 3.5 kV
+
|-
+
| Capillary temperature || 200 °C
+
|-
+
| Nebulizer gas || 1.5 l/min
+
|}
+
 
+
 
+
 
+
 
+
==LC-MS raw data==
+
 
+
===Smilacis Rhizoma LC-MS===
+
{{JCampViewer|Smilacis LC-MS.gz}}
+

Latest revision as of 16:33, 14 February 2011

LCMS
Data List
Names

[edit] TIC

LC-MS TIC of extracts

[edit] Analytical conditions (Positive mode)

Instrument Shimadzu LC-IT-TOF MS ESI
Column Waters Atlantis T3 (2.1 mm x 100 mm, 3 micron)
Column temperature 40 °C
Solvent A 5 mM Ammonium acetate solution
Solvent B acetonitrile
Gradient 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
Source voltage 4.5 kV
Capillary temperature 200 °C
Nebulizer gas 1.5 l/min

[edit] Analytical conditions (Negative mode)

Instrument Shimadzu LC-IT-TOF MS ESI
Column Waters Atlantis T3 (2.1 mm x 150 mm)
Column temperature 40 °C
Solvent A 5 mM Ammonium acetate solution
Solvent B acetonitrile
Gradient 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
Source voltage -3.5 kV
Capillary temperature 200 °C
Nebulizer gas 1.5 l/min
Personal tools
Namespaces

Variants
Actions
Navigation
metabolites
Toolbox