LCMS:Salviae miltiorhizae Radix

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(New page: ==TIC== LC-MS TIC of extracts ==Analytical conditions== {| class="wikitable" style="width:80%" |- | Instrument || Shimadzu LC-...)
 
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==TIC==
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{{CrudeDrug/TIC
[[Image:Salviae miltiorhizae LC-MS.jpg|thumb|700px|center|LC-MS TIC of extracts]]
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|TICFile=:Salviae miltiorhizae LC-MS
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}}
  
==Analytical conditions==
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{{CrudeDrug/LCMS/Pos
{| class="wikitable" style="width:80%"
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|PInstrument =Shimadzu LC-IT-TOF MS ESI
|-
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|PColumn    =Waters Atlantis T3 (2.1 mm x 100 mm, 3 micron)
| Instrument || Shimadzu LC-IT-TOF MS ESI (Positive ion mode)
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|PTemperature=40
|-
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|PSolventA  =5 mM Ammonium acetate solution
| Column || Waters Atlantis T3 (2.1 mm x 100 mm, 3 micron)
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|PSolventB  =acetonitrile
|-
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|PGradient  =10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
| Column Temperature|| 40℃ 
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|PVoltage    =4.5
|-
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|PCapillary  =200
| Solvent A || 5 mM Ammonium acetate solution
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|PGas        =1.5
|-
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}}
| Solvent B || acetonitrile
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|-
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| Gradient || 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
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|-
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| Source voltage ||  4.5 kV
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|-
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| Capillary temperature || 200 °C
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|-
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| Nebulizer gas || 1.5 l/min
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|}
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{| class="wikitable" style="width:80%"
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{{CrudeDrug/LCMS/Neg
|-
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|NInstrument =Shimadzu LC-IT-TOF MS ESI
| Instrument || Shimadzu LC-IT-TOF MS ESI (Negative ion mode)
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|NColumn    =Waters Atlantis T3 (2.1 mm x 150 mm)
|-
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|NTemperature=40
| Column || Waters Atlantis T3 (2.1 mm x 150 mm)
+
|NSolventA  =5 mM Ammonium acetate solution
|-
+
|NSolventB  =acetonitrile
| Column Temperature|| 40℃ 
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|NGradient  =10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
|-
+
|NVoltage    =-3.5
| Solvent A || 5 mM Ammonium acetate solution
+
|NCapillary  =200
|-
+
|NGas        =1.5
| Solvent B || acetonitrile
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}}
|-
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| Gradient || 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
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|-
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| Source voltage || – 3.5 kV
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|-
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| Capillary temperature || 200 °C
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|-
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| Nebulizer gas || 1.5 l/min
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|}
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==LC-MS raw data==
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===Salviae miltiorhizae Radix LC-MS===
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{{JCampViewer|Salviae miltiorhizae LC-MS.gz}}
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Revision as of 18:14, 10 February 2011

TIC

[[Image::Salviae miltiorhizae LC-MS.jpg|thumb|700px|center| LC-MS TIC of extracts]]

Analytical conditions (Positive mode)

Instrument Shimadzu LC-IT-TOF MS ESI
Column Waters Atlantis T3 (2.1 mm x 100 mm, 3 micron)
Column temperature 40 °C
Solvent A 5 mM Ammonium acetate solution
Solvent B acetonitrile
Gradient 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
Source voltage 4.5 kV
Capillary temperature 200 °C
Nebulizer gas 1.5 l/min

Analytical conditions (Negative mode)

Instrument Shimadzu LC-IT-TOF MS ESI
Column Waters Atlantis T3 (2.1 mm x 150 mm)
Column temperature 40 °C
Solvent A 5 mM Ammonium acetate solution
Solvent B acetonitrile
Gradient 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
Source voltage -3.5 kV
Capillary temperature 200 °C
Nebulizer gas 1.5 l/min
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