LCMS:Hochuekkito
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== 2D-Mass chromatogram == | == 2D-Mass chromatogram == | ||
| − | [[Image:Hochuekkito_L_LC.jpg|thumb | + | [[Image:Hochuekkito_L_LC.jpg|thumb|left|425px|Mass chromatographic fingerprints of HET using Atractylodis Lanceae Rhizoma]] |
| + | [[Image:Hochuekkito_LC.jpg|thumb|425px|Mass chromatographic fingerprints of HET using Atractylodis Rhizoma]] | ||
| − | + | {{CrudeDrug/LCMS/Neg | |
| − | + | |NInstrument =Shimadzu LC-IT-TOF MS ESI | |
| − | + | |NColumn =Waters Atlantis T3 (2.1 mm x 150 mm) | |
| − | + | |NTemperature=40 | |
| − | + | |NSolventA =5 mM Ammonium acetate solution | |
| − | | | + | |NSolventB =acetonitrile |
| − | + | |NGradient =10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) | |
| − | | | + | |NVoltage =-3.5 |
| − | | | + | |NCapillary =200 |
| − | + | |NGas =1.5 | |
| − | + | }} | |
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{| class="wikitable" | {| class="wikitable" | ||
| − | ! Retention Time (min) !! Compounds !!Molecular formula !!(M-H)- ions | + | ! Retention Time (min) !! Compounds !!Origin!! Molecular formula !! (M-H)- ions |
| − | + | {{#repeat:Mass/Fragmentation|5|{{{Fragmentation|}}}|&&}} | |
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|- | |- | ||
| − | ! | + | ! {{{1|8.33}}} |
| − | | [[ | + | | [[{{{2|Trihydroxyflavone-diglucopyranoside}}}]] ||{{{3|Aurantii Nobilis}}} || {{{4|C27H29O15}}} || {{{5|593.1495}}} |
|- | |- | ||
| − | ! | + | ! {{{1|8.59}}} |
| − | | [[ | + | | [[{{{2|Dihydroxyflavanone-glucopyranoside}}}]] ||{{{3|Glycyrrhizae}}} || {{{4|C21H21O9}}} || {{{5|417.1150}}} |
|} | |} | ||
Latest revision as of 16:22, 14 February 2011
| LCMS Data List |
Names |
[edit] 2D-Mass chromatogram
[edit] Analytical conditions (Negative mode)
| Instrument | Shimadzu LC-IT-TOF MS ESI |
| Column | Waters Atlantis T3 (2.1 mm x 150 mm) |
| Column temperature | 40 °C |
| Solvent A | 5 mM Ammonium acetate solution |
| Solvent B | acetonitrile |
| Gradient | 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) |
| Source voltage | -3.5 kV |
| Capillary temperature | 200 °C |
| Nebulizer gas | 1.5 l/min |
| Retention Time (min) | Compounds | Origin | Molecular formula | (M-H)- ions |
|---|---|---|---|---|
| 8.33 | Trihydroxyflavone-diglucopyranoside | Aurantii Nobilis | C27H29O15 | 593.1495 |
| 8.59 | Dihydroxyflavanone-glucopyranoside | Glycyrrhizae | C21H21O9 | 417.1150 |
