LCMS:Yokukansan

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'''Supplement data for the manuscript, Yamada et.al., “Ameliorative effects of Yokukansan on learning and memory deficits in olfactory bulbectomized mice.”'''
 
'''Supplement data for the manuscript, Yamada et.al., “Ameliorative effects of Yokukansan on learning and memory deficits in olfactory bulbectomized mice.”'''
 
[[Image:Yokukann1.jpg|thumb|800px|left|LC-MS TIC of extracts]]
 
[[Image:Yokukann1.jpg|thumb|800px|left|LC-MS TIC of extracts]]
 +
[[Image:Yokukann2.jpg|thumb|800px|left|LC-MS TIC of standard compounds]]
 +
 
{| class="wikitable" style="width:80%"
 
{| class="wikitable" style="width:80%"
 
|-  
 
|-  
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| Nebulizer gas || 1.5 l/min
 
| Nebulizer gas || 1.5 l/min
 
|}
 
|}
 
{| class="wikitable"
 
! Retention Time (min) !! Compounds !!Molecular formula !!(M-H)- ions
 
|-
 
! 12.60
 
| [[Liquiritin]] || C<sub>21</sub>H<sub>22</sub>O<sub>9</sub> || 417.1191
 
|-
 
! 12.85
 
| [[Liquiritin apioside]] || C<sub>26</sub>H<sub>22</sub>O<sub>9</sub> || 549.1614
 
|-
 
! 13.41
 
| [[Licoricesaponin G2]] || C<sub>42</sub>H<sub>62</sub>O<sub>17</sub> || 837.3914
 
|-
 
! 14.10
 
| [[Glycyrrhizin]] || C<sub>42</sub>H<sub>62</sub>O<sub>16</sub> || 821.3965
 
|-
 
! 14.26
 
| [[Licoricesaponin A3]] || C<sub>48</sub>H<sub>72</sub>O<sub>21</sub> || 983.4493
 
|-
 
! 14.47
 
| [[Licoricesaponin H2]] || C<sub>42</sub>H<sub>62</sub>O<sub>16</sub> || 821.3965
 
|-
 
! 14.92
 
| [[Licoricesaponin E2]] || C<sub>42</sub>H<sub>60</sub>O<sub>16</sub> || 819.3809
 
|-
 
! 16.12
 
| [[Licorice glycoside E]] || C<sub>35</sub>H<sub>35</sub>NO<sub>14</sub> || 692.1985
 
|}
 
 
 
{{JCampViewer|Glycyrrhizae_std_T3.JDX.gz}}
 
{{JCampViewer|Glycyrrhizae_std_T3.JDX.gz}}
 
 
== 2D-Mass chromatogram (Condition 2) ==
 
 
[[Image:Glycyrrhizae_Radix_LC2.jpg]]
 
{| class="wikitable" style="width:80%"
 
|-
 
| Instrument || Shimadzu LC-IT-TOF MS ESI (Negative ion mode)
 
|-
 
| Column || Waters Symmetry C18(2.1 mm x 150 mm)
 
|-
 
| Column Temperature|| 40℃ 
 
|-
 
| Solvent A || 0.1% Formic acid solution
 
|-
 
| Solvent B || acetonitrile
 
|-
 
| Gradient || 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
 
|-
 
| Source voltage || – 3.5 kV
 
|-
 
| Capillary temperature || 200 °C
 
|-
 
| Nebulizer gas || 1.5 l/min
 
|}
 
 
{| class="wikitable"
 
! Retention Time (min) !! Compounds !!Molecular formula !!(M-H)- ions
 
|-
 
! 11.48
 
| [[Liquiritin]] || C<sub>21</sub>H<sub>22</sub>O<sub>9</sub> || 417.1191
 
|-
 
! 11.65
 
| [[Liquiritin apioside]] || C<sub>26</sub>H<sub>22</sub>O<sub>9</sub> || 549.1614
 
|-
 
! 14.84
 
| [[Licorice glycoside E]] || C<sub>35</sub>H<sub>35</sub>NO<sub>14</sub> || 692.1985
 
|-
 
! 16.28
 
| [[Licoricesaponin A3]] || C<sub>48</sub>H<sub>72</sub>O<sub>21</sub> || 983.4493
 
|-
 
! 18.59
 
| [[Licoricesaponin E2]] || C<sub>42</sub>H<sub>60</sub>O<sub>16</sub> || 819.3809
 
|-
 
! 18.71
 
| [[Licoricesaponin G2]] || C<sub>42</sub>H<sub>62</sub>O<sub>17</sub> || 837.3914
 
|-
 
! 19.21
 
| [[Glycyrrhizin]] || C<sub>42</sub>H<sub>62</sub>O<sub>16</sub> || 821.3965
 
|-
 
! 20.19
 
| [[Licoricesaponin H2]] || C<sub>42</sub>H<sub>62</sub>O<sub>16</sub> || 821.3965
 
|}
 

Revision as of 12:48, 16 December 2010

Yokukansan LC-MS Data

Supplement data for the manuscript, Yamada et.al., “Ameliorative effects of Yokukansan on learning and memory deficits in olfactory bulbectomized mice.”

LC-MS TIC of extracts
LC-MS TIC of standard compounds
Instrument Shimadzu LC-IT-TOF MS ESI (Positive ion mode)
Column Waters Atlantis T3 (2.1 mm x 100 mm, 3 micron)
Column Temperature 40℃
Solvent A 5 mM Ammonium acetate solution
Solvent B acetonitrile
Gradient 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
Source voltage 4.5 kV
Capillary temperature 200 °C
Nebulizer gas 1.5 l/min
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