LCMS:Glycyrrhizae Radix
From Metabolomics.JP
(Difference between revisions)
| Line 2: | Line 2: | ||
[[Image:Glycyrrhizae_Radix_LC1.jpg]] | [[Image:Glycyrrhizae_Radix_LC1.jpg]] | ||
{| class="wikitable" style="width:80%" | {| class="wikitable" style="width:80%" | ||
| + | |- | ||
| + | | Instrument || Shimadzu LC-IT-TOF MS ESI (Negative ion mode) | ||
|- | |- | ||
| Column || Waters Atlantis T3 (2.1 mm x 150 mm) | | Column || Waters Atlantis T3 (2.1 mm x 150 mm) | ||
| Line 12: | Line 14: | ||
|- | |- | ||
| Gradient || 5% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) | | Gradient || 5% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) | ||
| + | |- | ||
| + | | Source voltage || – 3.5 kV | ||
| + | |- | ||
| + | | Capillary temperature || 200 °C | ||
| + | |- | ||
| + | | Nebulizer gas || 1.5 l/min | ||
|} | |} | ||
{| class="wikitable" | {| class="wikitable" | ||
| − | |||
! Retention Time (min) !! Compounds !!Molecular formula !!(M-H)- ions | ! Retention Time (min) !! Compounds !!Molecular formula !!(M-H)- ions | ||
|- | |- | ||
! 12.60 | ! 12.60 | ||
| − | | Liquiritin || C<sub>21</sub>H<sub>22</sub>O<sub>9</sub> || 417.1191 | + | | [[Liquiritin]] || C<sub>21</sub>H<sub>22</sub>O<sub>9</sub> || 417.1191 |
|- | |- | ||
! 12.85 | ! 12.85 | ||
| − | | Liquiritin apioside || C<sub>26</sub>H<sub>22</sub>O<sub>9</sub> || | + | | [[Liquiritin apioside]] || C<sub>26</sub>H<sub>22</sub>O<sub>9</sub> || 549.1614 |
| − | + | |- | |
| − | + | ! 13.41 | |
| + | | [[Licoricesaponin G2]] || C<sub>42</sub>H<sub>62</sub>O<sub>17</sub> || 837.3914 | ||
| + | |- | ||
| + | ! 14.10 | ||
| + | | [[Glycyrrhizin]] || C<sub>42</sub>H<sub>62</sub>O<sub>16</sub> || 821.3965 | ||
| + | |- | ||
| + | ! 14.26 | ||
| + | | [[Licoricesaponin A3]] || C<sub>48</sub>H<sub>72</sub>O<sub>21</sub> || 983.4493 | ||
| + | |- | ||
| + | ! 14.47 | ||
| + | | [[Licoricesaponin H2]] || C<sub>42</sub>H<sub>62</sub>O<sub>16</sub> || 821.3965 | ||
| + | |- | ||
| + | ! 14.92 | ||
| + | | [[Licoricesaponin E2]] || C<sub>42</sub>H<sub>60</sub>O<sub>16</sub> || 819.3809 | ||
| + | |- | ||
| + | ! 16.12 | ||
| + | | [[Licorice glycoside E]] || C<sub>35</sub>H<sub>35</sub>NO<sub>14</sub> || 692.1985 | ||
|} | |} | ||
[[Image:Glycyrrhizae_Radix_LC2.jpg]] | [[Image:Glycyrrhizae_Radix_LC2.jpg]] | ||
{| class="wikitable" style="width:80%" | {| class="wikitable" style="width:80%" | ||
| + | |- | ||
| + | | Instrument || Shimadzu LC-IT-TOF MS ESI (Negative ion mode) | ||
|- | |- | ||
| Column || Waters Symmetry C18(2.1 mm x 150 mm) | | Column || Waters Symmetry C18(2.1 mm x 150 mm) | ||
| Line 39: | Line 64: | ||
|- | |- | ||
| Gradient || 5% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) | | Gradient || 5% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) | ||
| + | |- | ||
| + | | Source voltage || – 3.5 kV | ||
| + | |- | ||
| + | | Capillary temperature || 200 °C | ||
| + | |- | ||
| + | | Nebulizer gas || 1.5 l/min | ||
| + | |} | ||
| + | |||
| + | {| class="wikitable" | ||
| + | ! Retention Time (min) !! Compounds !!Molecular formula !!(M-H)- ions | ||
| + | |- | ||
| + | ! 11.48 | ||
| + | | [[Liquiritin]] || C<sub>21</sub>H<sub>22</sub>O<sub>9</sub> || 417.1191 | ||
| + | |- | ||
| + | ! 11.65 | ||
| + | | [[Liquiritin apioside]] || C<sub>26</sub>H<sub>22</sub>O<sub>9</sub> || 549.1614 | ||
| + | |- | ||
| + | ! 14.84 | ||
| + | | [[Licorice glycoside E]] || C<sub>35</sub>H<sub>35</sub>NO<sub>14</sub> || 692.1985 | ||
| + | |- | ||
| + | ! 16.28 | ||
| + | | [[Licoricesaponin A3]] || C<sub>48</sub>H<sub>72</sub>O<sub>21</sub> || 983.4493 | ||
| + | |- | ||
| + | ! 18.59 | ||
| + | | [[Licoricesaponin E2]] || C<sub>42</sub>H<sub>60</sub>O<sub>16</sub> || 819.3809 | ||
| + | |- | ||
| + | ! 18.71 | ||
| + | | [[Licoricesaponin G2]] || C<sub>42</sub>H<sub>62</sub>O<sub>17</sub> || 837.3914 | ||
| + | |- | ||
| + | ! 19.21 | ||
| + | | [[Glycyrrhizin]] || C<sub>42</sub>H<sub>62</sub>O<sub>16</sub> || 821.3965 | ||
| + | |- | ||
| + | ! 20.19 | ||
| + | | [[Licoricesaponin H2]] || C<sub>42</sub>H<sub>62</sub>O<sub>16</sub> || 821.3965 | ||
|} | |} | ||
Revision as of 19:01, 7 December 2009
甘草水抽出物の2D-Mass chromatogram
| Instrument | Shimadzu LC-IT-TOF MS ESI (Negative ion mode) |
| Column | Waters Atlantis T3 (2.1 mm x 150 mm) |
| Column Temperature | 40℃ |
| Solvent A | 5 mM Ammonium acetate solution |
| Solvent B | acetonitrile |
| Gradient | 5% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) |
| Source voltage | – 3.5 kV |
| Capillary temperature | 200 °C |
| Nebulizer gas | 1.5 l/min |
| Retention Time (min) | Compounds | Molecular formula | (M-H)- ions |
|---|---|---|---|
| 12.60 | Liquiritin | C21H22O9 | 417.1191 |
| 12.85 | Liquiritin apioside | C26H22O9 | 549.1614 |
| 13.41 | Licoricesaponin G2 | C42H62O17 | 837.3914 |
| 14.10 | Glycyrrhizin | C42H62O16 | 821.3965 |
| 14.26 | Licoricesaponin A3 | C48H72O21 | 983.4493 |
| 14.47 | Licoricesaponin H2 | C42H62O16 | 821.3965 |
| 14.92 | Licoricesaponin E2 | C42H60O16 | 819.3809 |
| 16.12 | Licorice glycoside E | C35H35NO14 | 692.1985 |
| Instrument | Shimadzu LC-IT-TOF MS ESI (Negative ion mode) |
| Column | Waters Symmetry C18(2.1 mm x 150 mm) |
| Column Temperature | 40℃ |
| Solvent A | 0.1% Formic acid solution |
| Solvent B | acetonitrile |
| Gradient | 5% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) |
| Source voltage | – 3.5 kV |
| Capillary temperature | 200 °C |
| Nebulizer gas | 1.5 l/min |
| Retention Time (min) | Compounds | Molecular formula | (M-H)- ions |
|---|---|---|---|
| 11.48 | Liquiritin | C21H22O9 | 417.1191 |
| 11.65 | Liquiritin apioside | C26H22O9 | 549.1614 |
| 14.84 | Licorice glycoside E | C35H35NO14 | 692.1985 |
| 16.28 | Licoricesaponin A3 | C48H72O21 | 983.4493 |
| 18.59 | Licoricesaponin E2 | C42H60O16 | 819.3809 |
| 18.71 | Licoricesaponin G2 | C42H62O17 | 837.3914 |
| 19.21 | Glycyrrhizin | C42H62O16 | 821.3965 |
| 20.19 | Licoricesaponin H2 | C42H62O16 | 821.3965 |
