LCMS:Gardeniae Fructus
From Metabolomics.JP
(Difference between revisions)
m (moved Gardeniae Fructus LC-MS to LCMS:Gardeniae Fructus) |
Revision as of 14:50, 14 February 2011
TIC
Analytical conditions (Positive mode)
| Instrument | Shimadzu LC-IT-TOF MS ESI |
| Column | Waters Atlantis T3 (2.1 mm x 100 mm, 3 micron) |
| Column temperature | 40 °C |
| Solvent A | 5 mM Ammonium acetate solution |
| Solvent B | acetonitrile |
| Gradient | 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) |
| Source voltage | 4.5 kV |
| Capillary temperature | 200 °C |
| Nebulizer gas | 1.5 l/min |
Analytical conditions (Negative mode)
| Instrument | Shimadzu LC-IT-TOF MS ESI |
| Column | Waters Atlantis T3 (2.1 mm x 150 mm) |
| Column temperature | 40 °C |
| Solvent A | 5 mM Ammonium acetate solution |
| Solvent B | acetonitrile |
| Gradient | 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) |
| Source voltage | -3.5 kV |
| Capillary temperature | 200 °C |
| Nebulizer gas | 1.5 l/min |
