LCMS:Albizzae Cortex
From Metabolomics.JP
(Difference between revisions)
| Line 4: | Line 4: | ||
{{CrudeDrug/LCMS/Pos | {{CrudeDrug/LCMS/Pos | ||
| − | | | + | |PInstrument =Shimadzu LC-IT-TOF MS ESI |
| − | | | + | |PColumn =Waters Atlantis T3 (2.1 mm x 100 mm, 3 micron) |
| − | | | + | |PTemperature=40 |
| − | | | + | |PSolvent A =5 mM Ammonium acetate solution |
| − | | | + | |PSolvent B =acetonitrile |
| − | | | + | |PGradient =10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) |
| − | | | + | |PVoltage =4.5 |
| − | | | + | |PCapillary =200 |
| − | | | + | |PGas =1.5 |
}} | }} | ||
{{CrudeDrug/LCMS/Neg | {{CrudeDrug/LCMS/Neg | ||
| − | | | + | |NInstrument =Shimadzu LC-IT-TOF MS ESI |
| − | | | + | |NColumn =Waters Atlantis T3 (2.1 mm x 150 mm) |
| − | | | + | |NTemperature=40 |
| − | | | + | |NSolvent A =5 mM Ammonium acetate solution |
| − | | | + | |NSolvent B =acetonitrile |
| − | | | + | |NGradient =10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) |
| − | | | + | |NVoltage =-3.5 |
| − | | | + | |NCapillary =200 |
| − | | | + | |NGas =1.5 |
}} | }} | ||
Revision as of 13:14, 10 February 2011
TIC
Analytical conditions (Positive mode)
| Instrument | Shimadzu LC-IT-TOF MS ESI |
| Column | Waters Atlantis T3 (2.1 mm x 100 mm, 3 micron) |
| Column temperature | 40 °C |
| Solvent A | {{{PSolventA}}} |
| Solvent B | {{{PSolventB}}} |
| Gradient | 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) |
| Source voltage | 4.5 kV |
| Capillary temperature | 200 °C |
| Nebulizer gas | 1.5 l/min |
Analytical conditions (Negative mode)
| Instrument | Shimadzu LC-IT-TOF MS ESI |
| Column | Waters Atlantis T3 (2.1 mm x 150 mm) |
| Column temperature | 40 °C |
| Solvent A | {{{NSolventA}}} |
| Solvent B | {{{NSolventB}}} |
| Gradient | 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) |
| Source voltage | -3.5 kV |
| Capillary temperature | 200 °C |
| Nebulizer gas | 1.5 l/min |
