LCMS:Yokukansan
From Metabolomics.JP
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== Yokukansan LC-MS Data == | == Yokukansan LC-MS Data == | ||
| − | [[Image:Yokukann1.jpg|thumb|800px|left]] | + | Supplement data for the manuscript, Yamada et.al., “Ameliorative effects of Yokukansan on learning and memory deficits in olfactory bulbectomized mice.” |
| + | [[Image:Yokukann1.jpg|thumb|800px|left|LC-MS TIC of extracts]] | ||
{| class="wikitable" style="width:80%" | {| class="wikitable" style="width:80%" | ||
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Revision as of 12:44, 16 December 2010
Yokukansan LC-MS Data
Supplement data for the manuscript, Yamada et.al., “Ameliorative effects of Yokukansan on learning and memory deficits in olfactory bulbectomized mice.”
| Instrument | Shimadzu LC-IT-TOF MS ESI (Positive ion mode) |
| Column | Waters Atlantis T3 (2.1 mm x 100 mm, 3 micron) |
| Column Temperature | 40℃ |
| Solvent A | 5 mM Ammonium acetate solution |
| Solvent B | acetonitrile |
| Gradient | 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) |
| Source voltage | 4.5 kV |
| Capillary temperature | 200 °C |
| Nebulizer gas | 1.5 l/min |
| Retention Time (min) | Compounds | Molecular formula | (M-H)- ions |
|---|---|---|---|
| 12.60 | Liquiritin | C21H22O9 | 417.1191 |
| 12.85 | Liquiritin apioside | C26H22O9 | 549.1614 |
| 13.41 | Licoricesaponin G2 | C42H62O17 | 837.3914 |
| 14.10 | Glycyrrhizin | C42H62O16 | 821.3965 |
| 14.26 | Licoricesaponin A3 | C48H72O21 | 983.4493 |
| 14.47 | Licoricesaponin H2 | C42H62O16 | 821.3965 |
| 14.92 | Licoricesaponin E2 | C42H60O16 | 819.3809 |
| 16.12 | Licorice glycoside E | C35H35NO14 | 692.1985 |
2D-Mass chromatogram (Condition 2)
| Instrument | Shimadzu LC-IT-TOF MS ESI (Negative ion mode) |
| Column | Waters Symmetry C18(2.1 mm x 150 mm) |
| Column Temperature | 40℃ |
| Solvent A | 0.1% Formic acid solution |
| Solvent B | acetonitrile |
| Gradient | 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) |
| Source voltage | – 3.5 kV |
| Capillary temperature | 200 °C |
| Nebulizer gas | 1.5 l/min |
| Retention Time (min) | Compounds | Molecular formula | (M-H)- ions |
|---|---|---|---|
| 11.48 | Liquiritin | C21H22O9 | 417.1191 |
| 11.65 | Liquiritin apioside | C26H22O9 | 549.1614 |
| 14.84 | Licorice glycoside E | C35H35NO14 | 692.1985 |
| 16.28 | Licoricesaponin A3 | C48H72O21 | 983.4493 |
| 18.59 | Licoricesaponin E2 | C42H60O16 | 819.3809 |
| 18.71 | Licoricesaponin G2 | C42H62O17 | 837.3914 |
| 19.21 | Glycyrrhizin | C42H62O16 | 821.3965 |
| 20.19 | Licoricesaponin H2 | C42H62O16 | 821.3965 |
