LCMS:Hochuekkito
From Metabolomics.JP
(Difference between revisions)
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[[Image:Hochuekkito_LC.jpg|thumb|425px|Mass chromatographic fingerprints of HET using Atractylodis Rhizoma]] | [[Image:Hochuekkito_LC.jpg|thumb|425px|Mass chromatographic fingerprints of HET using Atractylodis Rhizoma]] | ||
− | {| | + | {{CrudeDrug/LCMS/Neg |
− | + | |NInstrument =Shimadzu LC-IT-TOF MS ESI | |
− | + | |NColumn =Waters Atlantis T3 (2.1 mm x 150 mm) | |
− | | | + | |NTemperature=40 |
− | + | |NSolventA =5 mM Ammonium acetate solution | |
− | | | + | |NSolventB =acetonitrile |
− | | | + | |NGradient =10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) |
− | + | |NVoltage =-3.5 | |
− | + | |NCapillary =200 | |
− | | | + | |NGas =1.5 |
− | + | }} | |
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{| class="wikitable" | {| class="wikitable" |
Revision as of 17:33, 10 February 2011
2D-Mass chromatogram
Analytical conditions (Negative mode)
Instrument | Shimadzu LC-IT-TOF MS ESI |
Column | Waters Atlantis T3 (2.1 mm x 150 mm) |
Column temperature | 40 °C |
Solvent A | 5 mM Ammonium acetate solution |
Solvent B | acetonitrile |
Gradient | 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) |
Source voltage | -3.5 kV |
Capillary temperature | 200 °C |
Nebulizer gas | 1.5 l/min |
Retention Time (min) | Compounds | Origin | Molecular formula | (M-H)- ions |
---|---|---|---|---|
8.33 | Trihydroxyflavone-diglucopyranoside | Aurantii Nobilis | C27H29O15 | 593.1495 |
8.59 | Dihydroxyflavanone-glucopyranoside | Glycyrrhizae | C21H21O9 | 417.1150 |