LCMS:Alismatis Rhizoma
From Metabolomics.JP
(Difference between revisions)
| Line 22: | Line 22: | ||
|NSolventB =acetonitrile | |NSolventB =acetonitrile | ||
|NGradient =10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) | |NGradient =10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) | ||
| − | |NVoltage = | + | |NVoltage =?3.5 |
|NCapillary =200 | |NCapillary =200 | ||
|NGas =1.5 | |NGas =1.5 | ||
}} | }} | ||
Revision as of 14:47, 10 February 2011
TIC
File:Alismatis Rhizoma LC-MS.jpg
LC-MS TIC of extracts
Analytical conditions (Positive mode)
| Instrument | Shimadzu LC-IT-TOF MS ESI |
| Column | Waters Atlantis T3 (2.1 mm x 100 mm, 3 micron) |
| Column temperature | 40 °C |
| Solvent A | 5 mM Ammonium acetate solution |
| Solvent B | acetonitrile |
| Gradient | 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) |
| Source voltage | 4.5 kV |
| Capillary temperature | 200 °C |
| Nebulizer gas | 1.5 l/min |
Analytical conditions (Negative mode)
| Instrument | Shimadzu LC-IT-TOF MS ESI |
| Column | Waters Atlantis T3 (2.1 mm x 150 mm) |
| Column temperature | 40 °C |
| Solvent A | 5 mM Ammonium acetate solution |
| Solvent B | acetonitrile |
| Gradient | 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) |
| Source voltage | ?3.5 kV |
| Capillary temperature | 200 °C |
| Nebulizer gas | 1.5 l/min |
